Col3a1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, R, WB
Recommend Dilution	RIA. Western blot. ELISA: ≥ 1/200. Immunflourescence: 1/80. Immunohistochemistry on Frozen and Paraffin Embedded Tissues: ≥ 1/500. Pretreatment: After de-waxing the tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: 60 minutes at RT or 2-8°C over night. Positive Control: Rat skin or liver.
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified Collagen type III from Rat skin.
Specificity	Rat Collagen type III: 100%. Rat Collagen I: < 0.1% Rat Collagen type V: < 10% Rat Elastin: < 0.1% (determined by solid phase RIA at 1/50 dilution).
Formulation	PBS without BSA or preservatives State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.1 ml distilled water. For further dilutions use appropriate antibody diluent.
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	collagen, type III, alpha 1
Database Link	Entrez Gene 12825 Mouse UniProt ID P08121
Background	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body.
Synonyms	COL3A1
Reference Data

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