Fibronectin Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, R
Recommend Dilution	IF: 1:40. IHC: 1:200 - 1:600 for paraffin sections, 60 min at RT or 2-8°C over night. ELISA. RIA. Positive control: Chicken skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Reactivity	Chicken
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified fibronectin from chicken plasma
Specificity	Chicken fibronectin 100%, chicken collagen type I, III and V < 0.1% (RIA at 1:10000 dilution). This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.
Formulation	State: Purified State: Lyophilized Ig fraction
Reconstitution Method	Reconstitute with 0.5 ml distilled water, for further dilution use appropriate antibody diluent.
Concentration	lot specific
Purification	Ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P11722
Background	Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.
Synonyms	FN1, Cold-insoluble globulin, CIG
Reference Data

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