HNF6 (ONECUT1) Mouse Monoclonal Antibody [Clone ID: OTI4F12]

Specifications

Product Data
Clone Name	OTI4F12
Applications	IF, IHC, WB
Recommend Dilution	WB 1:500, IHC 1:50, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 85-198 of human HNF6 (NP_004489) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	50.8 kDa
Gene Name	one cut homeobox 1
Database Link	NP_004489 Entrez Gene 15379 MouseEntrez Gene 25231 RatEntrez Gene 3175 Human UniProt ID Q9UBC0
Background	The protein encoded by HNF6 is Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a varietyof gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
Synonyms	HNF-6; HNF6; HNF6A
Reference Data
Protein Families	ES Cell Differentiation/IPS, Transcription Factors
Protein Pathways	Maturity onset diabetes of the young

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