LDHA Mouse Monoclonal Antibody [Clone ID: OTI2C11]

Specifications

Product Data
Clone Name	OTI2C11
Applications	IHC, IP, WB
Recommend Dilution	WB 1:2000, IHC 1:50, IP 2ug/500ul
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human LDHA (NP_005557) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	36.5 kDa
Gene Name	lactate dehydrogenase A
Database Link	NP_005557 Entrez Gene 16828 MouseEntrez Gene 3939 Human UniProt ID P00338
Background	The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq]
Synonyms	GSD11; HEL-S-133P; LDHM; PIG19
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cysteine and methionine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism

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