Aminoacylase 1 (ACY1) Mouse Monoclonal Antibody [Clone ID: OTI2D3]

Specifications

Product Data
Clone Name	OTI2D3
Applications	FC, IHC, WB
Recommend Dilution	WB 1:200~500, IHC 1:150, FLOW 1:100
Reactivity	Human, Dog, Rat, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human ACY1(NP_000657) produced in HEK293 cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	45.7 kDa
Gene Name	aminoacylase 1
Database Link	NP_000657 Entrez Gene 109652 MouseEntrez Gene 300981 RatEntrez Gene 476602 DogEntrez Gene 95 Human UniProt ID Q03154
Background	This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq]
Synonyms	ACY-1; ACY1D; HEL-S-5
Reference Data
Protein Families	Protease
Protein Pathways	Arginine and proline metabolism, Metabolic pathways

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