USP10 Mouse Monoclonal Antibody [Clone ID: OTI2E1]

Specifications

Product Data
Clone Name	OTI2E1
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human USP10(NP_005144) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	87 kDa
Gene Name	ubiquitin specific peptidase 10
Database Link	NP_005144 Entrez Gene 22224 MouseEntrez Gene 307905 RatEntrez Gene 479625 DogEntrez Gene 718471 MonkeyEntrez Gene 9100 Human UniProt ID Q14694
Background	Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. [provided by RefSeq]
Synonyms	UBPO
Reference Data
Protein Families	Druggable Genome, Protease

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