PFD6 (PFDN6) Mouse Monoclonal Antibody [Clone ID: OTI4E10]

Specifications

Product Data
Clone Name	OTI4E10
Applications	FC, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150, FLOW 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 2-129 of human PFDN6(NP_055075) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	14.4 kDa
Gene Name	prefoldin subunit 6
Database Link	NP_055075 Entrez Gene 14976 MouseEntrez Gene 309629 RatEntrez Gene 10471 Human UniProt ID O15212
Background	PFDN6 is a subunit of the heteromeric prefoldin complex that chaperones nascent actin (see MIM 102560) and alpha- and beta-tubulin (see MIM 602529 and MIM 191130, respectively) chains pending their transfer to the cytosolic chaperonin containing TCP1 (MIM 186980) (CCT) complex (Hansen et al., 1999 [PubMed 10209023]). [supplied by OMIM]
Synonyms	H2-KE2; HKE2; KE-2; PFD6
Reference Data
Protein Families	Stem cell - Pluripotency

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