NMT2 Mouse Monoclonal Antibody [Clone ID: OTI2A5]

Specifications

Product Data
Clone Name	OTI2A5
Applications	IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:150
Reactivity	Human, Rat, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human NMT2(NP_004799) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	56.8 kDa
Gene Name	N-myristoyltransferase 2
Database Link	NP_004799 Entrez Gene 18108 MouseEntrez Gene 291318 RatEntrez Gene 9397 Human UniProt ID O60551
Background	N-myristoyltransferase (NMT) catalyzes the reaction of N-terminal myristoylation of many signaling proteins. It transfers myristic acid from myristoyl coenzyme A to the amino group of a protein's N-terminal glycine residue. Biochemical evidence indicates the presence of several distinct NMTs, varying in apparent molecular weight and /or subcellular distribution. The predicted 498-amino acid of human NMT2 protein shares 77% and 96% sequence identity with human NMT1 and mouse Nmt2 comprise two distinct families of N-myristoyltransferases. [provided by RefSeq]
Reference Data
Protein Families	Druggable Genome

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