RUNX1T1 Mouse Monoclonal Antibody [Clone ID: OTI1H1]

Specifications

Product Data
Clone Name	OTI1H1
Applications	IF, WB
Recommend Dilution	WB 1:2000, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human RUNX1T1(NP_783553) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	63 kDa
Gene Name	RUNX1 partner transcriptional co-repressor 1
Database Link	NP_783553 Entrez Gene 12395 MouseEntrez Gene 362489 RatEntrez Gene 862 Human UniProt ID Q06455
Background	This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Synonyms	AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2
Reference Data
Protein Families	Transcription Factors
Protein Pathways	Acute myeloid leukemia, Pathways in cancer

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