UBC6e (UBE2J1) Mouse Monoclonal Antibody [Clone ID: OTI3D1]

Specifications

Product Data
Clone Name	OTI3D1
Applications	FC, IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:150, FLOW 1:100
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human UBE2J1(NP_057105) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	35 kDa
Gene Name	ubiquitin conjugating enzyme E2 J1
Database Link	NP_057105 Entrez Gene 56228 MouseEntrez Gene 297961 RatEntrez Gene 474993 DogEntrez Gene 701100 MonkeyEntrez Gene 51465 Human UniProt ID Q9Y385
Background	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
Synonyms	CGI-76; HSPC153; HSPC205; HSU93243; NCUBE-1; NCUBE1; UBC6; UBC6E; Ubc6p
Reference Data
Protein Families	Transmembrane
Protein Pathways	Parkinson's disease, Ubiquitin mediated proteolysis

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