GCLC Mouse Monoclonal Antibody [Clone ID: OTI1A3]

Specifications

Product Data
Clone Name	OTI1A3
Applications	IHC, WB
Recommend Dilution	WB 1:200~1000, IHC 1:150
Reactivity	Human, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human GCLC(NP_001489) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	72.6 kDa
Gene Name	glutamate-cysteine ligase catalytic subunit
Database Link	NP_001489 Entrez Gene 14629 MouseEntrez Gene 25283 RatEntrez Gene 710508 MonkeyEntrez Gene 2729 Human UniProt ID P48506
Background	Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction. [provided by RefSeq, Oct
Synonyms	GCL; GCS; GLCL; GLCLC
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Glutathione metabolism, Metabolic pathways

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