GADD45A Mouse Monoclonal Antibody [Clone ID: OTI2B7]

Specifications

Product Data
Clone Name	OTI2B7
Applications	WB
Recommend Dilution	WB 1:4000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human GADD45A(NP_001915) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	18.2 kDa
Gene Name	growth arrest and DNA damage inducible alpha
Database Link	NP_001915 Entrez Gene 13197 MouseEntrez Gene 25112 RatEntrez Gene 1647 Human UniProt ID P24522
Background	This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
Synonyms	DDIT1; GADD45
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Cell cycle, MAPK signaling pathway, p53 signaling pathway

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