Epoxide hydrolase (EPHX1) Mouse Monoclonal Antibody [Clone ID: OTI3F10]

Specifications

Product Data
Clone Name	OTI3F10
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150
Reactivity	Human, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 21-230 of human EPHX1 (NP_000111) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	52.8 kDa
Gene Name	epoxide hydrolase 1
Database Link	NP_000111 Entrez Gene 13849 MouseEntrez Gene 25315 RatEntrez Gene 700180 MonkeyEntrez Gene 2052 Human UniProt ID P07099
Background	Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2008]
Synonyms	EPHX; EPOX; HYL1; MEH
Reference Data
Protein Families	Druggable Genome, Protease
Protein Pathways	Metabolism of xenobiotics by cytochrome P450

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