COX2 (PTGS2) Mouse Monoclonal Antibody [Clone ID: OTI5F12]

Specifications

Product Data
Clone Name	OTI5F12
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 18-260 of human PTGS2 (NP_000954) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	67.2 kDa
Gene Name	prostaglandin-endoperoxide synthase 2
Database Link	NP_000954 Entrez Gene 19225 MouseEntrez Gene 29527 RatEntrez Gene 5743 Human UniProt ID P35354
Background	Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
Synonyms	COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; PHS-2
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Arachidonic acid metabolism, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway

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