Osteopontin (SPP1) Mouse Monoclonal Antibody [Clone ID: OTI5E4]

Specifications

Product Data
Clone Name	OTI5E4
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 17-314 of human SPP1(NP_001035147) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	33.7 kDa
Gene Name	secreted phosphoprotein 1
Database Link	NP_001035147 Entrez Gene 6696 Human UniProt ID P10451
Background	The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Synonyms	BNSP; BSPI; ETA-1; OPN
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway

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