SPI1 Mouse Monoclonal Antibody [Clone ID: OTI1F3]

Specifications

Product Data
Clone Name	OTI1F3
Applications	WB
Recommend Dilution	WB 1:2000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human SPI1(NP_003111) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	30.9 kDa
Gene Name	Spi-1 proto-oncogene
Database Link	NP_003111 Entrez Gene 20375 MouseEntrez Gene 366126 RatEntrez Gene 6688 Human UniProt ID P17947
Background	This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Synonyms	OF; PU.1; SFPI1; SPI-1; SPI-A
Reference Data
Protein Families	Transcription Factors
Protein Pathways	Acute myeloid leukemia, Pathways in cancer

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