PTK9 (TWF1) Mouse Monoclonal Antibody [Clone ID: OTI5C12]

Specifications

Product Data
Clone Name	OTI5C12
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 140-384 of human TWF1(NP_002813) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	43.7 kDa
Gene Name	twinfilin actin binding protein 1
Database Link	NP_002813 Entrez Gene 19230 MouseEntrez Gene 315265 RatEntrez Gene 5756 Human UniProt ID Q12792
Background	This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008]
Synonyms	A6; PTK9
Reference Data
Protein Families	Druggable Genome, Protein Kinase

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