n-Myc (MYCN) Mouse Monoclonal Antibody [Clone ID: OTI2A2]

Specifications

Product Data
Clone Name	OTI2A2
Applications	WB
Recommend Dilution	WB 1:500
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 229-464 of human MYCN(NP_005369) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	49.4 kDa
Gene Name	MYCN proto-oncogene, bHLH transcription factor
Database Link	NP_005369 Entrez Gene 4613 Human UniProt ID P04198
Background	This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
Synonyms	bHLHe37; MODED; N-myc; NMYC; ODED
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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