Ribonuclease H2, subunit A (RNASEH2A) Mouse Monoclonal Antibody [Clone ID: OTI7H10]

Specifications

Product Data
Clone Name	OTI7H10
Applications	WB
Recommend Dilution	WB 1:500-1:2000
Reactivity	Human,Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human RNASEH2A (NP_006388) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
Predicted Protein Size	33.4 kDa
Gene Name	ribonuclease H2 subunit A
Database Link	NP_006388 Entrez Gene 69724 MouseEntrez Gene 10535 Human UniProt ID O75792
Background	The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.[provided by RefSeq, Aug 2009]
Synonyms	AGS4; JUNB; RNASEHI; RNHIA; RNHL; THSD8
Reference Data
Protein Pathways	DNA replication

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