Cd8a Rat Monoclonal Antibody [Clone ID: CT-CD8a]
CAT#: CL008APC
Cd8a rat monoclonal antibody, clone CT-CD8a, APC
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CNY 8,963.00
货期*
5周
规格
Specifications
Product Data | |
Clone Name | CT-CD8a |
Applications | FC |
Recommend Dilution | Flow Cytometry Analysis (see Protocols). |
Reactivity | Mouse |
Host | Rat |
Clonality | Monoclonal |
Specificity | This anti-mouse CD8a antigen monoclonal antibody recognizes the mouse CD8α chain. The α chain of CD8 associates with the CD8β chain to form a CD8α/βheterodimer that is expressed by the majority of thymocytes and by the MHC class I restricted subset of mature T cells1. Mouse CD8α can also form a CD8α/α chain homodimer on subsets of CD8 positive T cells. For this reason, antibodies specific for CD8a rather than CD8b are recommended for a rigorous delineation of CD8 positive cells. |
Formulation | 0.5 ml PBS and 0.1% sodium azide (NaN3). A highly purified grade of BSA has been added as a stabilizing protein to bring the final protein concentration to 4-5 mg/ml after conjugation. Label: APC State: Liquid purified IgG |
Concentration | lot specific |
Conjugation | APC |
Storage Condition | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be proteced from light. |
Gene Name | CD8 antigen, alpha chain |
Database Link | |
Background | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. |
Synonyms | CD8 alpha chain, CD8A, MAL |
Note | Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add ~0.5 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Results: Mouse Strain: BALB/c Cell Concentration: 1 x 10e6 cells per test Antibody Concentration Used: 0.5 μg/10e6 cells Isotypic Control: APC Rat IgG2a |
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