Itga4 Rat Monoclonal Antibody [Clone ID: R1-2]

Specifications

Product Data
Clone Name	R1-2
Applications	FC
Recommend Dilution	Flow cytometry (see protocol). Immunoprecipitation. Immunohistochemistry. (1,2,3)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Peyers Patch HEV binding lymphoma line (TK1)
Specificity	Antibody CL030FX reacts with a4 integrin (mouse CD49d), which helps to mediate cell-cell and cell-matrix interactions.
Formulation	PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. Label: FITC State: Liquid
Concentration	lot specific
Purification	Purified from ascitic fluid via Protein G Chromatography
Conjugation	FITC
Storage Condition	Store at 4°C. For long term storage, aliquot and freeze unused portion at -20°C in volumes appropriate for single usage. Avoid prolonged exposure to light.
Gene Name	integrin alpha 4
Database Link	Entrez Gene 16401 Mouse UniProt ID Q00651
Background	Integrin alpha 4 (also called CD49d) is a 150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin alpha 4 forms heterodimers with integrins beta 1 and beta 7. Integrin alpha 4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin alpha 4 promotes cell migration and inhibits cell spreading and contractility. Integrin alpha 4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin alpha 4 function has become an important target for drug discovery.
Synonyms	Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
Note	Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 106 cells, representing 1 test). 4. To each tube, add 1.0 µg* of CL030F per 106 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most flurochromes are light sensitive.) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: BALB/c Cell Concentration: 1x106 cells per tests Antibody Concentration Used: 1.0 µg/106 cells Isotypic Control: FITC Rat IgG2b Cell Source Percentage of cells stained above control: TK1 cell line 96.8% Thymus 45.6% Spleen 88.0% Bone Marrow 84.7% Strain Distribution by Flow Cytometry Analysis: Cell Concentration: 1x106 cells per tests Antibody Concentration Used:1.0 µg /106 cells Strains Tested: BALB/c, C57BL/6, C3H/He, CBA/J, AKR Positive: BALB/c, C57BL/6, C3H/He, CBA/J, AKR Negative: non
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.