Macrophages Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FN, IHC
Recommend Dilution	Cytotoxic Assays. Immunohistochemistry on Frozen and Paraffin Sections.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Specificity	CL194P is directed against Mouse Macrophages. This antiserum is adsorbed repeatedly with Mouse Red Blood Cells, thymocytes and spleen cells to remove most antibodies to antigens common among different cell types. This adsorption significantly improves cytotoxic specificity.
Formulation	State: Aff - Purified State: Liquid serum (0.2 μm filtered) without preservatives
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Synonyms	Macrophage marker
Note	Protocol: Test Type: For Cytotoxic Antibodies: Modified Colorimetric Microtiter Assay (Ref.1) Results: Antisera of this lot diluted 1/20 exhibits greater than 75% cytotoxicity on Mouse Macrophages. Antisera of this lot diluted 1/20 exhibits less than 10% cytotoxicity on Mouse thymocytes or splenocytes. For Agglutinating Antibodies: Antisera dilutions in RPMI-1640 incubated with target cells at 4-8°C for 1hr. Agglutination determined by microscopic observations. Results: Antisera of this lot strongly agglutinates Mouse Macrophages at a dilution of 1/50.
Reference Data

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