Basophils Mouse Monoclonal Antibody [Clone ID: 212H6.16]
CAT#: DDX0030B-100
Basophils mouse monoclonal antibody, clone 212H6.16
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CNY 5,775.00
货期*
4周
规格
Specifications
Product Data | |
Clone Name | 212H6.16 |
Applications | FC, IF |
Recommend Dilution | IHC, WB, Flow Cytometry. Applications tested: MACS- separation and flow cytometry, IHC frozen section. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | enriched-human tonsillar pDCs |
Specificity | Human basophils and mast cells. Species cross-reactivity: no-cross reactivity on mouse basophil. |
Formulation | Label: Biotin State: 100 μg in 200 μl/ 50 μg in 100 μl PBS 50% glycerol |
Concentration | lot specific |
Purification | QMA Hyper D Ion exchange chromatography |
Conjugation | Biotin |
Storage Condition | -20°C. KEEP CONTENTS STERILE: no preservative. Coupled antibodies: glycerol protects from freezing. |
Background | Basophils and mast cells are multifunctional hematopoietic effector cells involved in allergic and inflammatory reactions. Immediate allergy is caused by release of chemical mediators by these cell types. In human peripheral blood, 212H6.16 is an antibody specific to a yet non-identified cell-surface structure (~30KDa in reduced WB conditions and ~46KDa in non-reduced WB conditions) present on basophils. 212H6.16 does not induce basophil degranulation, and is therefore a valuable tool for the isolation and study of human basophils. Tonsillar epithelium and skin keratinocytes are also stained by 212H6 in situ: antigen expression in tonsil is restricted to cells lining the epithelial crypts. (Bendriss-Vermare N.et al, Leukocyte Typing VII, White Cell Differentiation Antigen MC13, part 9, myeloid cells, 425-30, 2001; Editor Mason) |
Synonyms | Basophil cells |
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