IL17 (IL17A) Mouse Monoclonal Antibody [Clone ID: 403D10.01]

Specifications

Product Data
Clone Name	403D10.01
Applications	FC, Neutralize
Recommend Dilution	DDX0330P-50 DDX0330P-100 Purified: FACS intracellular, Neutralization. DDX0330B-50 DDX0330B-100 Biotin: ELISA Detection. Usage recommendation: *This monoclonal antibody may be used between 1-10 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use.
Reactivity	Canine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	rhCytotoxic T Lymphocyte associated-Antigen 8 transfected COS-7 cells.
Specificity	Human IL-17A. Species cross-reactivity: Dog. Neutralizing activity: mAbs anti-IL17 suppress IL6 and IL8 secretion by rheumatoid synovial fibroblasts.
Formulation	Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol. State: Purified
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	interleukin 17A
Database Link	Entrez Gene 3605 Human UniProt ID Q16552
Background	IL-17 (cytotoxic T lymphocyte associated antigen 8) is a CD4+ T cell-derived cytokine that stimulates stromal cells and macrophages to secrete proinflammatory cytokines. To address a possible mechanism by which IL-17 may promote alloreactivity, we examined the influence of IL-17 on the differentiation and function of bone marrow-derived cells propagated in GM-CSF with or without IL-4 to promote dendritic cell (DC) growth. A minor proportion of CD11c+ DC expressed the IL-17R. IL-17 promoted the maturation of DC progenitors, as evidenced by increased cell surface expression of CD11c, costimulatory molecules (CD40, CD80, CD86), and MHC class II Ag, and allostimulatory capacity. IL-17 had a lesser effect on the phenotype and function of more fully differentiated myeloid DC. These findings suggest a role for IL-17 in allogeneic T cell proliferation that may be mediated in part via a maturation-inducing effect on DC. IL-17 appears to be a novel target for therapeutic intervention in allograft rejection. hIL17 stimulate epithelial, endothelial, and fibroblastic cells to secrete cytokines such as IL-6, IL-8, and G-CSF and PGE2. (Fossiez F et al, 1996 ; J. Exp. Med., 183:2593-2603 ; Fossiez F. et al, 1998 ; Int. Rev. Immunol., 16:541-551).
Synonyms	IL-17A, IL17, IL-17, CTLA8, CTLA-8
Reference Data

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