Langerin (CD207) Rat Monoclonal Antibody [Clone ID: 929F3.01]

Specifications

Product Data
Clone Name	929F3.01
Applications	FC, IF, IHC
Recommend Dilution	DDX0362P-50 / DDX0362P-100 Purified: IF, IHC formol-paraffin, Flow cytometry. DDX0362A488-50 / DDX0362A488-100 Alexa-fluor® 488: IF, IHC , Flow cytometry. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application.  *Coupled antibody: to maintain RT before use.
Reactivity	Human, Mouse, Porcine, Rat
Host	Rat
Clonality	Monoclonal
Immunogen	human Langerin-transfected eukaryotic cells
Specificity	Human Langerin. Species cross-reactivity: Rat, Mouse, Swine.
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol. Label: Alexa Fluor 488
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 488
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	CD207 molecule
Database Link	Entrez Gene 50489 Human UniProt ID Q9UJ71
Background	Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck’s granule. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin expression has not been reported outside the DC system. Human Langerin is involved in the processing of mycobacterial glycolipids leading to antigen presentation to CD1a-restricted T cells. Human Langerin binds the gp120 of HIV. (Valladeau J et al, 2002 J. Immunol, 168 : 782-792; Douillard P et al, 2005 J Invest Dermatol, 125: 983–994; Chorro L. et al, J Exp.Med., 2010, 206 (13), 3089-3100).
Synonyms	CLEC4K, Dendritic Cell Marker
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.