SEMA6A Mouse Monoclonal Antibody [Clone ID: 118E7]

Specifications

Product Data
Clone Name	118E7
Applications	FC
Recommend Dilution	DDX0463P-50 / DDX0463P-100 Purified: Surface Flow Cytometry. DDX0463A488-50 / DDX0463A488-100 Alexa-fluor®488: Surface Flow Cytometry. DDX0463A546-50 / DDX0463A546-100 Alexa-fluor®546: IF. DDX0463A647-50 / DDX0463A647-100 Alexa- fluor®647: (on request) IHC, Flow cytometry. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	human recombinant SEMA-6A
Specificity	Human SEMA-6A (epitope in extracellular domain).
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol. Label: Alexa Fluor 488
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 488
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	semaphorin 6A
Database Link	Entrez Gene 57556 Human UniProt ID Q9H2E6
Background	Semaphorins represent a large family composed of 19 secreted, GPI-linked or transmembrane members divided into 5 classes (3 to 7) and characterized by the presence of a 500 amino acid Sema domain in the Nterminus. Initially described for their role in axon guidance and apoptosis during neuronal development, some semaphorins like the class 4 regulate immune functions and are involved in organogenesis and angiogenesis. The transmembrane class 6 semaphorins include 4 members (SEMA-6A to 6D). SEMA-6A features an intracellular binding site for regulatory Evl protein. In the immune system, SEMA6A protein is restricted in vitro and in vivo to an activation status of Langerhans cells. (Gautier G et al., 2006 Am J Pathol., 168: 453-65).
Synonyms	Semaphorin-6A, Semaphorin VIA, Sema VIA, Semaphorin-6A-1, SEMA6A-1, SEMAQ, KIAA1368
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.