TLR8 Rat Monoclonal Antibody [Clone ID: 112H7.15]

Specifications

Product Data
Clone Name	112H7.15
Applications	FC
Recommend Dilution	DDX0483P-50 / DDX0483P-100 Purified: ImmunoHistoChemistry frozen sections, Western Blot, FACS intracellular. DDX0483A488-50 / DDX0483A488-100 Alexa-fluor®488: FACS intracellular. DDX0483A546-50 / DDX0483A546-100 Alexa-fluor®546: ImmunoFluorescence. DDX0483A647-50 / DDX0483A647-100 Alexa-fluor®647: FACS intracellular. DDX0483B-50 / DDX0483B-100 Biotin: Western Blot. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	Human recombinant TLR8.
Specificity	Human TLR8. Species cross- reactivity: Negative.
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol. Label: Alexa Fluor 647
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 647
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	toll like receptor 8
Database Link	Entrez Gene 51311 Human UniProt ID Q9NR97
Background	The Toll-Like Receptors (TLRs) represent a family of germline-encoded proteins, composed of C-terminal leucine-rich repeats (LRRs), and an N-terminal Toll/Interleukin-1 Receptor (TIR) domain. In humans, 10 TLRs, sharing high sequence homology, have been identified. TLRs are critical for the detection of pathogen-associated molecular patterns (PAMPs) by the innate immune system. LRRs recognize PAMPs, and signal transduction events, initiated by the TIR domain, lead to activation of transcription factors such as AP-1, IRFs and NFkB, and therefore expression of proinflammatory cytokines and costimulatory molecules. TLR7, TLR8 and TLR9 form a subgroup in the TLR family, because of a strong sequence homology and the nature of their ligands, which are nucleic acids or related molecules. TLR7 and TLR8 are triggered by GU-rich, single-stranded RNA (ssRNA) derived from viruses, or by synthetic small molecules mimicking ssRNA, such as imidazoquinolines. While TLR7 is expressed in lung, placenta and spleen, TLR8 expression is restricted to lung and peripheral blood leukocytes (PBLs). (Heil F. et al, 2003, Eur.J.Immunol, 33, 2987).
Synonyms	Toll-like receptor 8, UNQ249/PRO286
Reference Data

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