HIV-1 (gp41) Mouse Monoclonal Antibody [Clone ID: 425D11.01]

Specifications

Product Data
Clone Name	425D11.01
Applications	ELISA, FC, IF, Neutralize
Recommend Dilution	DDX1306P-50 / DDX1306P-100 Purified: Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition.  DDX1306A488-50 / DDX1306A488-100 Alexa- fluor®A488 (on request): Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition. DDX1306A546-50 / DDX1306A546-100 Alexa- fluor®546 (on request): Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition. DDX1306A647-50 / DDX1306A647-100 Alexa- fluor®647 (on request): Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition. DDX1306B-50 / DDX1306B-100 Biotin (on request): Blocking of HR1/HR2 complex formation, Cell-coated ELISA, FACS surface, ImmunoFluorescence, Neutralization, Inhibition.  Applications tested: Dawood R et al, AIDS. 27(5):717-730, 2013. Usage recommendation: *This monoclonal antibody may be used between 5-25 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using.
Reactivity	Human Immunodeficiency Virus 1
Host	Mouse
Clonality	Monoclonal
Immunogen	HR1-PID-HR2-transfected HEK 293 cells (HIV-1, 92BR025, Clade C).
Specificity	Conformational epitope formed by aa 531-560 (HR1) and 623-661 (HR2). Clone 425D11.01 referred to as clone 3 in the article.
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol. Label: Alexa Fluor 488
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 488
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Background	HIV-1-gp41 is a subunit of the envelope protein complex of HIV. HIV-1-gp41 is responsible for the fusion between the viral and the cell membranes and thus represents a target for HIV vaccines. Trimeric Env is composed of gp120, which is non-covalently associated with the membrane-anchored fusion protein gp41. HIV-1 gp120 binding to CD4 and co-receptor (CCR5 or CXCR4) induces conformational changes, resulting in gp41 exposure and in the production of fusion-intermediate conformation of gp41 (HR1 and HR2). 3 neutralizing anti-gp41 monoclonal antibodies were selected from mice immunized with 293T cells stably transfected with a construct expressing HR1 plus HR2. (Dawood R et al, AIDS. 27(5):717-730, 2013)
Synonyms	HIV1, HIV-I, Human immunodeficiency virus type 1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.