Prostate Specific Antigen (KLK3) Mouse Monoclonal Antibody [Clone ID: CHYH1]

Specifications

Product Data
Clone Name	CHYH1
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: In Sandwich ELISA this antibody can be used as a Capture antibody in combination with a Monoclonal tracer/detection antibody Clone CHYH2 (HRP conjugate Cat.-No DM1036HRP, TMB as substrate). Recommended Capture Coating Dose: 0.5-1.0 µg/well. Under the suggested conditions approximately 1 ng/ml of PSA in human serum can be detected with an assay range of 0-200 ng/ml.  Note: Matched pair antibodies are DM1035 (Clone CHYH1) and DM1036 (Clone CHYH2). Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections (ABC method). Western blot: 10 µg/ml allows visualization of 1 µg/lane Human PSA.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human Prostate Specific Antigen (PSA)
Specificity	DM1035 reacts with PSA. This antibody is reactive to normal prostate, benign prostate hyperplasia and prostate malignant carcinoma. It has not reacted with other normal tissues tested.
Formulation	0.01M PBS, pH 7.0 without preservatives State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with double distilled water to a final concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	kallikrein related peptidase 3
Database Link	Entrez Gene 354 Human UniProt ID P07288
Background	Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates two transcript variants encoding different isoforms. Additional transcript variants have been described, but it is unclear if these transcripts are normally expressed or if they are specific to benign or malignant tumors.
Synonyms	Prostate-specific antigen, Semenogelase, Gamma-seminoprotein, P-30 antigen
Reference Data

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