MCP1 (CCL2) Mouse Monoclonal Antibody [Clone ID: S101]

Specifications

Product Data
Clone Name	S101
Applications	ELISA, FN, WB
Recommend Dilution	ELISA: React with human MCP-1. Matched pair antibodies are available. Neutralizing: Tests on the ability to inhibit monocyte chemotaxis toward 1 nM recombinant human MCP-1 in blindwell chambers showed that the antibodies were particularly effective at blocking MCP-1 activity at a concentration of 0.5 µg/mL. It was also found that S-14 could inhibit the function of native MCP-1 at concentrations similar to inhibitory doses for recombinant MCP-1. Western Blot: Concentration of 0.1-1.0 µg/mL will allow visualization of 0.1 µg/lane of human MCP-1. Concentration of 5.0 µg/mL will allow visualization of 50 µg/lane of rabbit MCP-1.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant Human MCP-1
Specificity	This MCP1 antibody reacts with natural and recombinant Human MCP-1. Does not react with Human Interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1 beta (IL1 beta), serum Amyloid A (SAA) and epidermal growth factor (EGF).
Formulation	0.01M PBS, pH 7.2 without preservatives State: Azide Free State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 2
Database Link	Entrez Gene 6347 Human UniProt ID P13500
Background	Monocyte chemotactic and activating factor (MCAF) is also called monocyte chemotactic protein-1 (MCP-1) and chemokine (C-C motif) ligand 2 (CCL2). It is primarily secreted by monocytes, macrophages and dendritic cells. This cytokine displays chemotactic activity for monocytes, T-cells, and basophils, but not for neutrophils or eosinophils. MCAF causes the degranulation of basophils and mast cells, and augments the activity of monocyte and macrophage. MCAF plays an important role in inflammation, angiogenesis, autoimmune diseases, renal diseases, chronic infection and granuloma formation.
Synonyms	C-C motif chemokine 2, SCYA2, MCAF, Small-inducible cytokine A2, MCP-1, HC11, HC-11
Reference Data

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