PDPN Mouse Monoclonal Antibody [Clone ID: 18H5]

Specifications

Product Data
Clone Name	18H5
Applications	FC
Recommend Dilution	FACS Analysis: Use the antibody at ≤ 0.5 µg in 100 µl volume.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Podoplanin-expressing MDCK cells.
Specificity	This antibody reacts with Podoplanin (gp36).  The unconjugated monoclonal antibody will detect native Human Podoplanin in Western Blot and on the surface of Human LEC.
Formulation	PBS Label: PE State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	PE
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!  This product is photosensitive and should be protected from light.
Gene Name	podoplanin
Database Link	Entrez Gene 10630 Human UniProt ID Q86YL7
Background	Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
Synonyms	Glycoprotein 36, PA2.26 antigen, T1-alpha, Aggrus, PDPN, GP36, PSEC0003, PSEC0025
Reference Data

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