Pdpn Hamster Monoclonal Antibody [Clone ID: RTD4E10]

Specifications

Product Data
Clone Name	RTD4E10
Applications	IHC, IP, WB
Recommend Dilution	Immunohistochemistry on Frozen and Paraffin Sections: 1-10 µg/ml. Western blot: 1-10 µg/ml. The antibody will detect a 36-40 kDa band. Immunoprecipitation: 1-5 µg IgG per 1 ml lysate or reaction volume.
Reactivity	Mouse
Host	Hamster
Clonality	Monoclonal
Immunogen	Murine thymic stromal cell line
Specificity	The monoclonal antibody will detect Podoplanin on the surface of lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry. The antibody does not cross-react with Human Podoplanin.
Formulation	PBS, pH 7.4, without preservatives or stabilizers State: Purified State: Lyophilized purified IgG fraction from Cell Culture Supernatant
Reconstitution Method	Restore with sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	podoplanin
Database Link	Entrez Gene 14726 Mouse UniProt ID Q62011
Background	Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a Mr 38,000 membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.
Synonyms	Glycoprotein 36, PA2.26 antigen, T1-alpha, Aggrus, PDPN, GP36, PSEC0003, PSEC0025
Reference Data

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