MLH1 Mouse Monoclonal Antibody [Clone ID: G168-15]

Specifications

Product Data
Clone Name	G168-15
Applications	IHC
Recommend Dilution	Immunohistochemistry on formalin-fixed paraffin embedded sections: Dilute 1/25-1/50 in an ABC method (30 to 60 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil, colon carcinoma.
Reactivity	Hamster, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length recombinant MLH.
Specificity	The G168-15 antibody recognizes MLH1 80-85kDa. Cellular Localization: Nuclear
Formulation	State: Supernatant State: Liquid Supernatant containing Sodium Azide as preservative.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	mutL homolog 1
Database Link	Entrez Gene 4292 Human UniProt ID P40692
Background	The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.
Synonyms	DNA mismatch repair protein Mlh1, COCA2
Reference Data

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