Cystatin C (CST3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA. Western Blot.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Cystatin C from Human urine.
Specificity	The antibody recognizes Human Cystatin C. Other species not tested.
Formulation	Azide Free 0.05M Phosphate buffer, 0.1M NaCl, pH 7.2 State: Aff - Purified State: Lyophilized purified IgG fraction Preservative: None
Reconstitution Method	Restore with 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Concentration	1.0 mg/ml (after reconstitution)
Purification	Immunoaffinity Chromatography on a column with immobilized Human Cystatin C
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	cystatin C
Database Link	Entrez Gene 1471 Human UniProt ID P01034
Background	Cystatin C is a non-glycosilated basic single-chain protein consisting of 120 amino acids with a molecular weight of 13.36 kDa and is characterized by two disulfide bonds in the carboxy-terminal region. It belongs to the cystatins superfamilly which inactivates lysosomal cysteine proteinases, e.g. cathepsin B, H,.K, L and S. Imbalance between Cystatin C and cysteine proteinases is associated with inflammation, renal failure, cancer, Alzheimers disease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Its increased level has been found in patients with autoimune diseases, with colorectal tumors and in patients on dyalisis. Serum Cystatin C seems to be better marker of glomerular filtration rate than creatinine. On the other hand, low concentration of Cystatin C presents a risk factor for secondary cardiovascular events.
Synonyms	Cystatin C, Cystatin-3, CST3
Note	Quality Control Test: Indirect ELISA to determine titer of the antibody. SDS PAGE to determine purity of the antibody.
Reference Data

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