Neuroglobin (NGB) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Western Blot. Immunohistochemistry. ELISA (for determination of the titer): The titer of this antibody, measured by indirect ELISA,  is >1:100,000 for antibody concentration 1 mg/ml, 25 ng of antigen are coated per well, and  defined at a point of maximal decrease of the titration curve.
Reactivity	Canine, Human, Rat
Host	Chicken
Clonality	Polyclonal
Immunogen	Recombinant human neuroglobin. The Human Neuroglobin is created as a recombinant protein produced in E. coli. It is the 17 kDa protein containing 150 amino acid residues of the Human Neuroglobin and one extra AA, N-terminal methionin. The amino acid sequence of the recombinant human neuroglobin is 100% homologous with the amino acid sequence of the human neuroglobin.
Specificity	This antibody recognizes Neuroglobin.
Formulation	Azide Free,  0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2 State: Aff - Purified State: Lyophilized purified IgG fraction Preservative: None
Reconstitution Method	Restore with 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Concentration	1.0 mg/ml (after reconstitution)
Purification	Immunoaffinity chromatography on a column with immobilized recombinant Human Neuroglobin
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	neuroglobin
Database Link	Entrez Gene 58157 Human UniProt ID Q9NPG2
Background	Neuroglobin, a 151 amino acid reside protein, mainly expressed in vertebrate brain and retina, is a recently identified member of the globin superfamily. Augmenting O2 supply, neuroglobin promotes survival of neurons upon hypoxic injury, potentially limiting brain damage. Moreover, neuroglobin may be a novel oxidative stress-responsive sensor for signal transduction in the brain. Neuroglobin expression is increased by neuronal hypoxia in vitro and focal cerebral ischemia in vivo, and neuronal survival after hypoxia is reduced by inhibiting neuroglobin expression with an antisense oligodeoxynucleotide and enhanced by neuroglobin overexpression.
Synonyms	NGB
Note	Quality Control Test Indirect ELISA - to determine titer of the antibody. SDS PAGE - to determine purity of the antibody.
Reference Data

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