Lyve1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA (1-15 µg/ml). Western blot (1-5 µg/ml). FACS analysis (3-10 µg/ml). Immunofluorescence. Immunohistochemistry on Frozen Sections (1-5 µg/ml). For formalin-fixed, paraffin-embedded sections use the immunogen affinity purified antibody Cat.-No DP3513P.
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure recombinant Mouse soluble LYVE-1 (Ala24-Gly228) produced in insect cells (Cat.-No DA3524). This recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis).
Specificity	This antibody detetcs Mouse and Rat Lyve-1. This antibody is not reactive with Human LYVE-1.
Formulation	PBS, pH 7.2 without preservatives or stabilizers State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in sterile water/PBS to a concentration of 0.1-1.0 mg/ml.
Purification	Protein G Chromatography (+his tag depletetion)
Conjugation	Unconjugated
Storage Condition	The lyophilized IgG is stable at 2-8°C for one month and at -20°C for longer. When reconstituted the antibody is stable for at least six weeks at 2-8°C. For longer store in aliquots at -20°C. Avoid repeated freezing and thawing.
Gene Name	lymphatic vessel endothelial hyaluronan receptor 1
Database Link	Entrez Gene 114332 Mouse UniProt ID Q8BHC0
Background	LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212- esidue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE- molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymphspecific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Synonyms	LYVE1, CRSBP-1, CRSBP1, HAR, XLKD1
Reference Data

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