SLC18A3 / VACHT Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Immunofluorescence. Immunohistochemistry on Frozen Sections. Working Dilution: 1/5000-1/10,000 using FITC. Incubation Time: Overnight at 4°C. Recommended Positive Control: Frozen Sections of Rat small intestine.
Reactivity	Mouse, Rat
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic peptide from the C-terminal of Rat VAChT conjugated to BSA.
Specificity	The antiserum against the vesicular acetylcholine transporter is a unique Immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylchiolinesterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT). The antiserum recognizes VAChT both in the CNS and PNS of Rat and Mouse. Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes staining.
Formulation	State: Serum State: Lyophilized Undiluted Serum
Reconstitution Method	Dissolve in 50-100 µl distilled water, and dilute further with 0.1M PBS with 1% BSA and 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID O35304
Background	Vesicular Acetylcholine Transporter (VAChT), (~70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate. VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer's disease and other nervous system disorders.
Synonyms	VAChT, Vesicular acetylcholine transporter
Reference Data

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