Fgf9 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: (Direct): To detect mFGF-9 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2- 0.4 ng/well of recombinant mFGF-9. (Sandwich): To detect mFGF-9 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine FGF-9 (PP002P) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mFGF-9. Western Blot: To detect mFGF-9 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mFGF-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant mFGF-9.
Specificity	Recognises Fibroblast Growth Factor 9 (FGF-9).
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	fibroblast growth factor 9
Database Link	Entrez Gene 14180 Mouse UniProt ID P54130
Background	FGF9 is a heparin binding growth factor, which is a member of the FGF family of proteins. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF9 is produced mainly by neurons and may have a role in glial cell growth and differentiation during development; gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
Synonyms	Glia-activating factor, GAF, Fibroblast growth factor 9, HBGF9
Note	Centrifuge vial prior to opening!
Reference Data

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