Ifng Rabbit Polyclonal Antibody

CAT#: PP004P2

Ifng rabbit polyclonal antibody, Aff - Purified

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Applications ELISA, FN, WB
Recommend Dilution Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine IFN-gamma (1.00 ng/ml), a concentration of 0.05-0.1 µg/ml of this antibody is required.
Sandwich ELISA: To detect Mouse IFN-gamma by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with biotinylated anti-Murine IFN-gamma (Cat.-No PP004B1 or PP004B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIFN-gamma.
Western Blot: To detect Mouse IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) E.coli derived recombinant Murine IFN-gamma (Cat.-No PA007)
Specificity Detects Mouse Interferon gamma (IFNg).
Other species not tested.
Formulation PBS, pH 7.2 without additives
State: Aff - Purified
State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method Centrifuge prior to opening.
Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography employing immobilized Murine IFN-gamma matrix
Conjugation Unconjugated
Storage Condition Can be stored lyophilized at 2-8°C for 1 month or at -20°C long term.
Gene Name interferon gamma
Background Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
Synonyms IFN-gamma, gamma IFN
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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