Il1b Rabbit Polyclonal Antibody
CAT#: PP006B1
Il1b rabbit polyclonal antibody, Biotin
Conjugation: Unconjugated
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CNY 3,910.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | ELISA: To detect mIL-1 beta by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIL-1 beta. Western Blot: To detect mIL-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1 beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly pure (>98%) recombinant mIL-1 beta. |
Specificity | Detect Murine IL-1 beta. |
Formulation | PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized purified Ig fraction. Label: conjugated |
Reconstitution Method | Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity chromatography. |
Conjugation | Biotin |
Storage Condition | Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | interleukin 1 beta |
Database Link | |
Background | Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence. |
Synonyms | IL-1 beta, IL1B, IL1 beta, IL1F2, Catabolin |
Note | Centrifuge vial prior to opening! |
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