Il1b Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Mouse IL-1β (10 pg/ml), a concentration of 0.57-0.86 µg/ml of this antibody is required. Direct ELISA: To detect Mouse IL-1-beta by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant Mouse IL-1-beta. Sandwich ELISA: To detect Mouse IL-1β by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1β (Cat.-No PP006B1 or PP006B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1β. Western Blot: To detect Mouse IL-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse IL-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) E.coli derived recombinant Murine IL-1-beta. (Cat.-No PA009).
Specificity	This antibody recognizes Interleukin-1 beta / IL-1B. Other species not tested.
Formulation	PBS, pH 7.4 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with sterile water to a concentration of 0.1-1.0 mg/ml.
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using immobilized mIL-1β matrix.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	interleukin 1 beta
Database Link	Entrez Gene 16176 Mouse UniProt ID P10749
Background	Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
Synonyms	IL-1 beta, IL1B, IL1 beta, IL1F2, Catabolin
Note	Centrifuge vial prior to opening.
Reference Data

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