IL5 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-5 (0.45 ng/ml), a concentration of 0.05-0.09 µg/ml of this antibody is required. Sandwich ELISA: To detect Human IL-5 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotin conjugated antibody (Cat.-No PP1027B) as a detection antibody, allows the detection of 0.2-0.4 ng/well of recombinant Human IL-5. Western Blot: To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 0.25 µg/ml with an overnight incubation at 4°C. Optimal results for these conditions were achieved without antigen retrieval.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human IL-5 (Cat.-No PA078)
Specificity	This antibody reacts with Human Interleukin-5. Other species not tested.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing immobilized Human IL-5 matrix.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	interleukin 5
Database Link	Entrez Gene 3567 Human UniProt ID P05113
Background	Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70% amino acid sequence homology.
Synonyms	IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF
Note	Centrifuge vial prior to opening!
Reference Data

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