PDGF beta (PDGFB) (PDGF-BB) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human PDGF-BB by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1.0 µg/ml. This Biotinylated antibody allows the detection of at least 0.2 ng/well of recombinant hPDGF-BB. Sandwich ELISA: To detect Human PDGF-BB by Sandwich ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. This Biotinylated antibody, in conjunction with anti-Human PDGF-BB antibody (PP1062P1 or PP1062P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) Recombinant Human PDGF-B.
Specificity	Recognizes Human Platelet Derived Growth Factor BB (hPDGF-BB)
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	platelet derived growth factor subunit B
Database Link	Entrez Gene 5155 Human UniProt ID P01127
Background	Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B, the dimers being connected by disulfide bonds. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.
Synonyms	Platelet-derived growth factor subunit B, PDGF beta, PDGF B, PDGF-B, Platelet-derived growth factor beta, PDGF subunit B, Platelet-derived growth factor B chain, PDGF2, PDGF-2, c-Sis, SIS, Becaplermin
Note	Centrifuge vial prior to opening!
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma

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