SDF1 (CXCL12) (alpha) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: Using 100 μl/well antibody solution, a concentration of ~1.0 μg/ml is required. This Biotin conjugated antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human SDF1-alpha. Sandwich ELISA: Using 100 μl/well antibody solution, a concentration of 0.25-1.0 μg/ml is required. In conjunction with Purified Anti-Human SDF1-alpha (Cat.-No PP1067P) as a Capture antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human SDF1-alpha. Western Blot: 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human SDF1-alpha (Cat.-No PA126)
Specificity	This antibody detects Human Stromal Cell Derived Factor-1 alpha. Other species not tested.
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	C-X-C motif chemokine ligand 12
Database Link	Entrez Gene 6387 Human UniProt ID P48061
Background	SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.
Synonyms	Stromal cell-derived factor 1, SDF1A, SDF1B, IRH, hIRH, PBSF
Note	Centrifuge vial prior to opening.
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	Axon guidance, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Leukocyte transendothelial migration

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.