CXCL16 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Sandwich ELISA: To detect Human CXCL16 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antibody, in conjunction with a Biotinylated anti-Human CXCL16 (cat.no. PP1204B1 or PP1204B2) as a Detection antibody, allows the detection of 0.2-0.4 ng/well of recombinant Human CXCL16. Western Blot: To detect Human CXCL16 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. The detection limit for recombinant Human CXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: The antibody stained FFPE Sections of Human colorectal adenocarcinoma tissue. An HRP-labelled polymer detection system was used with a DAB chromogen. Antigen retrieval is not required for these conditions. Working Dilution: 0.5 µg/ml, overnight incubation at 4°C.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) E.coli derived 10.1 kDa recombinant Human CXCL16 (Cat.-No AR31138PU)
Specificity	This antibody recognizes Human CXCL16. Other species not tested.
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml
Purification	Affinity Chromatography employing immobilized hCXCL16 matrix
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at -20°C. Following reconstitution store undiluted at 2-8°C for two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-X-C motif chemokine ligand 16
Database Link	Entrez Gene 58191 Human UniProt ID Q9H2A7
Background	CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. It is a type I membrane protein (Potential). Also exists as a soluble form. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.
Synonyms	C-X-C motif chemokine 16, Small-inducible cytokine B16, SR-PSOX, SCYB16, SRPSOX
Note	Centrifuge vial prior to opening!
Reference Data

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