GDF3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Indirect ELISA: To detect hGDF-3 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant hGDF-3. Sandwich ELISA: To detect hGDF-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GDF-3 (PP1227B1, PP1227B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGDF-3. Western blot: To detect hGDF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDF-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant hGDF-3 (human GDF-3)
Specificity	This antibody is specific to GDF3.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (Sterile filtered) purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	tore lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	growth differentiation factor 3
Database Link	Entrez Gene 9573 Human UniProt ID Q9NR23
Background	GDF3 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. The function of this protein is unknown, but expression studies suggest it may be involved in regulation of the adult lymphatic and erythroid systems and embryonic development.
Synonyms	Growth/differentiation factor 3, GDF-3
Note	Centrifuge vial prior to opening.
Reference Data

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