WNT1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Indirect ELISA: To detect hWNT-1 by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hWNT-1. Sandwich ELISA: To detect hWNT-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human WNT-1 (PP1231B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hWNT-1. Western Blot: To detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 62.5 ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant human WNT-1
Specificity	The antibody recognizes Human WNT-1. Other species not tested.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (sterile filtered) purified antibody
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity chromatography employing immobilized hWNT-1 matrix
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	Wnt family member 1
Database Link	Entrez Gene 7471 Human UniProt ID P04628
Synonyms	Proto-oncogene protein Wnt-1, INT1, INT-1
Reference Data

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