p27 KIP 1 (CDKN1B) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Suitable for ELISA, Immunoprecipitation, Immunoblotting and other immunological methods requiring high titer and specificity. Recommended Dilutions: This product was assayed by immunoblot and found to be reactive against p27 from cell lysates and E.coli produced fusion proteins at a dilution of 1:2000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Expect a band at ~27 kDa in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human, rat and mouse p27. This antisera is suitable for Immmunoprecipitation of in vitro translated protein and cell lysates (C212, 3T3-L1) and will co-precipitate associated proteins.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Full length human p27 protein.
Specificity	This product was Antiserum will specifically react with a p27 protein from human tissue. No reaction was observed against other related tumor suppressor proteins. No reactivity is observed with p27 from mouse. Reactivity against p27 from other species is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. State: Serum State: Liquid (sterile filtered) purified Ig fraction.
Concentration	lot specific
Purification	Prepared from monospecific antiserum by delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Gene Name	cyclin-dependent kinase inhibitor 1B
Database Link	Entrez Gene 1027 Human UniProt ID P46527
Background	p27 Kip is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21. p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Synonyms	p27Kip1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.