CIITA Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/5,000-1/25,000. Western Blot: 1/500-1/3,000. Note: This product has been assayed by immunoblot against CIITA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 is suggested for this product. Best Results are obtained using 0.03% (v/v) Triton X-100 IN BUFFERS. Do not supplement buffers with Tween-20 detergent.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide corresponding to a region near the C-terminal end (amino acids 726-742) of the human CIITA gene conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Cross reactivity against CIITA from other sources may occur but has not been specifically determined.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative. State: Ig Fraction State: Liquid (sterile filtered) purified IgG fraction.
Concentration	lot specific
Purification	Multi-step process.
Conjugation	Unconjugated
Storage Condition	Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Gene Name	class II, major histocompatibility complex, transactivator
Database Link	Entrez Gene 4261 Human UniProt ID P33076
Background	The transactivator CIITA regulates basal and interferon-induced expression of MHC class II genes. CIITA restores expression of all MHC II gene expression in mutant cells and corrects regulatory defects of those genes. Activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. CIITA may act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter.
Synonyms	MHC2TA
Reference Data

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