Collagen III (COL3A1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for native (non-denaturating, non-dissociating) PAGE and Western blotting for highly sensitive qualitative analysis. Recommended Dilutions: ELISA: 1/5,000-1/50,000. Western blot: 1/1,000-1/10,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/50-1/200.
Reactivity	Bovine, Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Collagen type III purified from Human and Bovine placenta.
Specificity	This product has been prepared by Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III Collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross reaction of anti-Collagen antibodies with other Human serum proteins or non-collagen extracellular matrix proteins is negligible.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 State: Aff - Purified State: Liquid (sterile filtered) purified Ig fraction Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. For extended storage, mix with an equal volume of glycerol. Avoid repeated freezing and thawing.
Gene Name	collagen type III alpha 1 chain
Database Link	Entrez Gene 1281 Human UniProt ID P02461
Background	Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type-specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens for immunization are purified from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type'-specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
Synonyms	COL3A1
Reference Data

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